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halotag7 insert  (Addgene inc)


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    Structured Review

    Addgene inc halotag7 insert
    a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
    Halotag7 Insert, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/halotag7 insert/product/Addgene inc
    Average 91 stars, based on 5 article reviews
    halotag7 insert - by Bioz Stars, 2026-03
    91/100 stars

    Images

    1) Product Images from "Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging"

    Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging

    Journal: bioRxiv

    doi: 10.1101/2022.10.24.513449

    a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
    Figure Legend Snippet: a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

    Techniques Used: Construct, Expressing, Control, Incubation, Imaging, Fluorescence, Plasmid Preparation



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    halotag7 insert  (Addgene inc)
    91
    Addgene inc halotag7 insert
    a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
    Halotag7 Insert, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/halotag7 insert/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    halotag7 insert - by Bioz Stars, 2026-03
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

    Journal: bioRxiv

    Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging

    doi: 10.1101/2022.10.24.513449

    Figure Lengend Snippet: a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

    Article Snippet: The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group, Heidelberg) as templates.

    Techniques: Construct, Expressing, Control, Incubation, Imaging, Fluorescence, Plasmid Preparation